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| Under Graduate Degree |
Year module |
NQF level: 7 |
Credits: 12 |
| Module presented in English |
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| Pre-requisite: MIB2604 |
Co-requisite: Any two of the following: MIB3701, MIB3702, MIB3703 |
| Purpose: of this module, students will have been exposed to a range of microbiology laboratory equipment such as basic measurement of bacterial colonies using colony counters to more advanced instruments such as thermocyclers and flow cytometers. They will be able to follow a laboratory protocol and analyse the data that is obtained from such a protocol. Students will also be able to describe laboratory techniques used in recombinant DNA experiments. Students will review techniques to recycle water and determine some industrial applications of microorganisms including the biological treatment of waste and xenobiotic compounds.
Students will receive practical training and demonstrations involving the following instruments: micropipettes, laminar flow hood, biological safety hoods, inverted and standard microscopes, incubators for cell culture, incubators for bacterial growth, colony counters, dry blocks, bench top and floor standing centrifuges, autoclaves, pH meters, refrigerators and freezers (4ﾰ, -20ﾰ and -80ﾰC), water deionizer, hot plates, stirrers, balances, flow cytometer, standard and real time PCR cyclers, ELISA washer and spectrophotometer, advanced bacteriological identity analyzer, DNA sequencer, electrophoresis tanks and power packs and gel documentation system. Gas and liquid chromatography and inductively coupled plasma systems will be demonstrated in relation to water quality analysis.
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